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  2. USMLE Step 1
  3. Biochemistry: Glycolysis
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USMLE Step 1

High-yield board prep across pharmacology, biochemistry, and microbiology.

Medicine
PShared by Priya Nair
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Biochemistry: Glycolysis

The 10-step glucose pathway, its regulation, and ATP yield.

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Content

45 total questions

MCQ: 8
Fill-in: 11
Flashcards: 16
Pair match: 10
Biochemistry: Glycolysis – Flashcards
Flashcards

Card #1

Where in the cell does glycolysis occur?

Card #2

Net ATP yield of glycolysis per glucose?

Card #3

Rate-limiting enzyme of glycolysis?

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Biochemistry: Glycolysis – Fill-in-the-blank
Fill in the Blank
  1. 1. Glycolysis produces a net of ___ ATP molecules per glucose.

    Answer:2also: two

    Hint: Four made, two invested.

  2. 2. The rate-limiting enzyme of glycolysis is ___.

    Answer:phosphofructokinase-1also: PFK-1, PFK1, phosphofructokinase 1

    Hint: It phosphorylates fructose-6-phosphate.

  3. 3. The end product of glycolysis is two molecules of ___.

    Answer:pyruvatealso: pyruvic acid

    Hint: Enters the TCA cycle aerobically.

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Biochemistry: Glycolysis – MCQ
Multiple Choice Questions
  1. 1. The rate-limiting and most important regulated enzyme of glycolysis is:

    • Phosphofructokinase-1
    • Hexokinase
    • Pyruvate kinase
    • Aldolase
  2. 2. The most potent allosteric activator of PFK-1 is:

    • Fructose-2,6-bisphosphate
    • Citrate
    • ATP
    • Glucose-6-phosphate
  3. 3. Which best distinguishes glucokinase from hexokinase?

    • Glucokinase has a high Km and is not inhibited by glucose-6-phosphate
    • Glucokinase has a low Km and high affinity for glucose
    • Glucokinase is found in all tissues
    • Glucokinase is inhibited by insulin
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Biochemistry: Glycolysis – Pair match
Pair Match
Hexokinase↔Low-Km first step; inhibited by glucose-6-phosphate
Glucokinase↔High-Km hepatic isozyme induced by insulin
Phosphofructokinase-1↔Rate-limiting step; activated by fructose-2,6-bisphosphate
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